The Lebanon Valley Railroad is a railroad line between the cities of Harrisburg and Reading, both in the American state of Pennsylvania. The line opened on 18 January 1858. The road was acquired by the Reading Company on 20 March 1858, which put them in direct competition with the Pennsylvania Railroad for the Philadelphia to Harrisburg route. The line now belongs to Norfolk Southern Railway and is part of its Harrisburg Line running between Harrisburg and Philadelphia.
The 1870 maps actually display the Lebanon Valley Railroad as running between Easton and Bethlehem and the Pennsylvania Railroad running between Harrisburg and Reading.