October 28 Monday – The Clemens family left Florence for Rome. The trip took 8 hours and they arrived at 4:30 PM [MTNJ 2: 235]. The party stayed at the Hotel d’Allemania. Sam noted the cost of the rooms, three coffees, one beefsteak and three “table d’hotes” (communal table, full-course meal) totaling 48.25 francs, paid at 5 PM.

There does not seem to be any information of what route they took.

Bædeker Central Italy and Rome (1869) 

Route 6: From Florence to Rome by Siena, Orvieto and Viterbo page 24

Route 8 : From Florence to Rome by Arezzo, Perugia and Foligno Page 44